CHM 3430 California State Polytechnic Pomona Solid Phase Microextraction Lab Report: Chemistry Answers 2021

CHM 3430 California State Polytechnic Pomona Solid Phase Microextraction Lab Report: Chemistry Answers 2021

CHM 3430 California State Polytechnic Pomona Solid Phase Microextraction Lab Report: Chemistry Answers 2021

Question Title:

CHM 3430 California State Polytechnic Pomona Solid Phase Microextraction Lab Report

Full Question:

I’m working on a chemistry multi-part question and need guidance to help me understand better.

Multi-elemental speciation analysis of organometallic compounds of mercury, lead and tin in two natural waters samples were carried out by headspace-solid phase microextraction followed by GC MS. Read the attached literature (CHM 3430L Lab Unit 3 Assignment 2 Literature.pdf) and watch the instructional videos carefully, and answer the following questions:What is SPME? How is the SPME performed? (4 pts)A buffer with pH 5.3 was prepared by using 0.2 M acetic acid and 0.2 M acetate. How many mL of acetic acid and sodium acetate are required to prepare 100.0 mL of such a buffer. The pKa for acetic acid is 4.76. Show your work. Hint: you will need to set the volume of each as x and y. (8 pts)What fibers were used in SPME? (4 pts)What is the stationary phase for HP-5MS column used in this experiment? Is it polar or nonpolar? (4 pts)Was GC injection a split or splitless one? (2 pts)Was an isothermal program or temperature programming used in GC? Why? (4 pts)What was the mobile phase used? What was the flow rate of the mobile phase? (4 pts)Spike recovery test is an important way to determine if the response to the analysis is decreased or increased by something else in the sample. How was the recovery experiment performed in this literature? (4 pts)What type of method was used in the literature to enhance the extraction efficiency (4 pts)If the organometallic compound has a concentration of 0.5%, what type of injection techniques should be used? (2 pts)

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